plasmid ymtq2 δ 11  (Addgene inc)

Journal: iScience

Article Title: A fast method to distinguish between fermentative and respiratory metabolisms in single yeast cells

doi: 10.1016/j.isci.2023.108767

Figure Lengend Snippet: Yeast strains and plasmids

Article Snippet: A second purification step was performed in a 1% agarose gel using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific) to remove the original vector used as template in the PCR reaction. pDRF1-GW yAT1.03 and ymTq2 Δ 11 were digested with Nhe I HF and Not I HF (New England Biolabs, Ipswich, Massachusetts, USA) and the opened vector pDRF1-GW and insert ymTq2 Δ 11 purified from a 1% agarose gel using the GeneJET Gel Extraction Kit.

Techniques: Plasmid Preparation

Journal: iScience

Article Title: A fast method to distinguish between fermentative and respiratory metabolisms in single yeast cells

doi: 10.1016/j.isci.2023.108767

Figure Lengend Snippet:

Article Snippet: A second purification step was performed in a 1% agarose gel using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific) to remove the original vector used as template in the PCR reaction. pDRF1-GW yAT1.03 and ymTq2 Δ 11 were digested with Nhe I HF and Not I HF (New England Biolabs, Ipswich, Massachusetts, USA) and the opened vector pDRF1-GW and insert ymTq2 Δ 11 purified from a 1% agarose gel using the GeneJET Gel Extraction Kit.

Techniques: Virus, Recombinant, Gel Extraction, Purification, Plasmid Preparation, Software, DNA Sequencing, Membrane, Transformation Assay

Journal: iScience

Article Title: A fast method to distinguish between fermentative and respiratory metabolisms in single yeast cells

doi: 10.1016/j.isci.2023.108767

Figure Lengend Snippet: Yeast strains and plasmids

Article Snippet: The plasmid ymTq2 Δ 11 generated in this study have been deposited to [Addgene, pDRF1-GW ymTq2-d11, #20549].

Techniques: Plasmid Preparation

Journal: iScience

Article Title: A fast method to distinguish between fermentative and respiratory metabolisms in single yeast cells

doi: 10.1016/j.isci.2023.108767

Figure Lengend Snippet:

Article Snippet: The plasmid ymTq2 Δ 11 generated in this study have been deposited to [Addgene, pDRF1-GW ymTq2-d11, #20549].

Techniques: Virus, Recombinant, Gel Extraction, Purification, Plasmid Preparation, Software, DNA Sequencing, Membrane, Transformation Assay

Journal: iScience

Article Title: A fast method to distinguish between fermentative and respiratory metabolisms in single yeast cells

doi: 10.1016/j.isci.2023.108767

Figure Lengend Snippet: Yeast strains and plasmids

Article Snippet: The plasmid ymTq2 Δ 11 generated in this study have been deposited to [Addgene, pDRF1-GW ymTq2-d11, #20549].

Techniques: Plasmid Preparation

Journal: iScience

Article Title: A fast method to distinguish between fermentative and respiratory metabolisms in single yeast cells

doi: 10.1016/j.isci.2023.108767

Figure Lengend Snippet:

Article Snippet: The plasmid ymTq2 Δ 11 generated in this study have been deposited to [Addgene, pDRF1-GW ymTq2-d11, #20549].

Techniques: Virus, Recombinant, Gel Extraction, Purification, Plasmid Preparation, Software, DNA Sequencing, Membrane, Transformation Assay

Journal: iScience

Article Title: A fast method to distinguish between fermentative and respiratory metabolisms in single yeast cells

doi: 10.1016/j.isci.2023.108767

Figure Lengend Snippet: Yeast strains and plasmids

Article Snippet: CEN.PK113-5D , ymTq2 pDRF1-GW , ymTq2 , ymTq2 , D. Botman , Addgene #118453.

Techniques: Plasmid Preparation

Journal: iScience

Article Title: A fast method to distinguish between fermentative and respiratory metabolisms in single yeast cells

doi: 10.1016/j.isci.2023.108767

Figure Lengend Snippet:

Article Snippet: CEN.PK113-5D , ymTq2 pDRF1-GW , ymTq2 , ymTq2 , D. Botman , Addgene #118453.

Techniques: Virus, Recombinant, Gel Extraction, Purification, Plasmid Preparation, Software, DNA Sequencing, Membrane, Transformation Assay

Journal: iScience

Article Title: A fast method to distinguish between fermentative and respiratory metabolisms in single yeast cells

doi: 10.1016/j.isci.2023.108767

Figure Lengend Snippet: Yeast strains and plasmids

Article Snippet: CEN.PK113-5D + ymTq2 pDRF1-GW , D. Botman , Addgene #118453.

Techniques: Plasmid Preparation

Journal: iScience

Article Title: A fast method to distinguish between fermentative and respiratory metabolisms in single yeast cells

doi: 10.1016/j.isci.2023.108767

Figure Lengend Snippet:

Article Snippet: CEN.PK113-5D + ymTq2 pDRF1-GW , D. Botman , Addgene #118453.

Techniques: Virus, Recombinant, Gel Extraction, Purification, Plasmid Preparation, Software, DNA Sequencing, Membrane, Transformation Assay